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Open Access Highly Accessed Commentary

Blood from ‘junk’: the LTR chimeric transcript Pu.2 promotes erythropoiesis

Kyle R Upton1 and Geoffrey J Faulkner12*

Author Affiliations

1 Mater Research Institute – University of Queensland, TRI Building, 4102 Brisbane, QLD, Australia

2 School of Biomedical Sciences, University of Queensland, 4072 Brisbane, QLD, Australia

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Mobile DNA 2014, 5:15  doi:10.1186/1759-8753-5-15

Published: 9 May 2014

Abstract

Transposable elements (TEs) are a prominent feature of most eukaryotic genomes. Despite rapidly accumulating evidence for the role of TE-driven insertional mutagenesis and structural variation in genome evolution, few clear examples of individual TEs impacting biology via perturbed gene regulation are available. A recent report describes the discovery of an alternative promoter for the murine erythroid transcription factor Pu.1. This promoter is located in an ORR1A0 long terminal repeat (LTR) retrotransposon intronic to Pu.1 and is regulated by the Krüppel-like factors KLF1 and KLF3. Expression of the resultant chimeric transcript, called Pu.2, spontaneously induces erythroid differentiation in vitro. These experiments illustrate how transcription factor binding sites spread by retrotransposition have the potential to impact networks encoding key biological processes in the host genome.

Keywords:
LTR retrotransposon; Transposable element; ORR1A0; KLF1; KLF3; Erythroid; Pu.1; Pu.2