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Open Access Research

Striking structural dynamism and nucleotide sequence variation of the transposon Galileo in the genome of Drosophila mojavensis

Mar Marzo13*, Xabier Bello2, Marta Puig14, Xulio Maside2 and Alfredo Ruiz1

Author Affiliations

1 Departament de Genètica i de Microbiologia, Universitat Autònoma de Barcelona, Bellaterra, Catalunya, 08193, Spain

2 Departamento de Anatomía Patolóxica e Ciencias Forenses; Grupo de Medicina Xenómica, Centro de Investigación en Medicina Molecular e Enfermedades Crónicas (CIMUS), Universidade de Santiago de Compostela, Galicia, 15782, Spain

3 Present address: School of Biomedical Sciences, Queen’s Medical Centre, University of Nottingham, Nottingham, NG7 2UH, UK

4 Present address: Unitat de Genòmica, Institut de Biotecnologia i Biomedicina, Universitat Autònoma de Barcelona, Bellaterra, Catalunya, 08193, Spain

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Mobile DNA 2013, 4:6  doi:10.1186/1759-8753-4-6

Published: 4 February 2013

Abstract

Background

Galileo is a transposable element responsible for the generation of three chromosomal inversions in natural populations of Drosophila buzzatii. Although the most characteristic feature of Galileo is the long internally-repetitive terminal inverted repeats (TIRs), which resemble the Drosophila Foldback element, its transposase-coding sequence has led to its classification as a member of the P-element superfamily (Class II, subclass 1, TIR order). Furthermore, Galileo has a wide distribution in the genus Drosophila, since it has been found in 6 of the 12 Drosophila sequenced genomes. Among these species, D. mojavensis, the one closest to D. buzzatii, presented the highest diversity in sequence and structure of Galileo elements.

Results

In the present work, we carried out a thorough search and annotation of all the Galileo copies present in the D. mojavensis sequenced genome. In our set of 170 Galileo copies we have detected 5 Galileo subfamilies (C, D, E, F, and X) with different structures ranging from nearly complete, to only 2 TIR or solo TIR copies. Finally, we have explored the structural and length variation of the Galileo copies that point out the relatively frequent rearrangements within and between Galileo elements. Different mechanisms responsible for these rearrangements are discussed.

Conclusions

Although Galileo is a transposable element with an ancient history in the D. mojavensis genome, our data indicate a recent transpositional activity. Furthermore, the dynamism in sequence and structure, mainly affecting the TIRs, suggests an active exchange of sequences among the copies. This exchange could lead to new subfamilies of the transposon, which could be crucial for the long-term survival of the element in the genome.

Keywords:
Transposable element; Drosophila mojavensis; Evolution; Terminal inverted repeat; Phylogeny; Genomics