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Open Access Correction

Correction: Reliable transgene-independent method for determining sleeping beauty transposon copy numbers

Orsolya Kolacsek1, Virág Krízsik1, Anita Schamberger1, Zsuzsa Erdei1, Ágota Apáti1, György Várady1, Lajos Mátés2, Zsuzsanna Izsvák23, Zoltán Ivics23, Balázs Sarkadi1 and Tamás I Orbán1*

Author Affiliations

1 Membrane Research Group of the Hungarian Academy of Sciences, Semmelweis University and National Blood Center, Budapest, Hungary

2 Mobile DNA Group, Max-Delbrück Center for Molecular Medicine, Berlin, Germany

3 University of Debrecen, Debrecen, Hungary

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Mobile DNA 2013, 4:11  doi:10.1186/1759-8753-4-11


The electronic version of this article is the complete one and can be found online at: http://www.mobilednajournal.com/content/4/1/11


Received:20 December 2012
Accepted:20 December 2012
Published:15 March 2013

© 2013 Kolacsek et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Correction

After the publication of our article [1], the authors noticed two typing errors in the ’Methods’ part of the original article:

– in Table two, the correct sequence of RPPH1 / Probe is 2 nucleotides longer at the 3’ end, so it is: CACTCCCATGTCCCTT ;

– in the penultimate sentence of the 'Quantitative real-time PCR' section, the concentrations of the primers and the probe were switched so the correct sentence should be: "Final concentrations of primers and probes were 900 and 250 nM, respectively."

The authors apologize for these unintended mistakes in the text.

Competing interests

The authors declare that they have no competing interests.

Authors’ contributions

OK established the HEK clones, OK and VK optimized the real-time PCR and performed copy number measurements, AS, ZE and ÁA established the HUES9 clones, GV helped in FACS measurements, LM measured copy numbers in HeLa clones, ZsI and ZI gave technical help and advices with the SB transposon work, BS provided financial support and discussed the data and TIO designed the overall strategy, analyzed the data and wrote the paper. All authors read and approved the final manuscript.

References

  1. Kolacsek O, Krízsik V, Schamberger A, Erdei Z, Apáti Á, Várady G, Mátés L, Izsvák Z, Ivics Z, Sarkadi B, Orbán TI: Reliable transgene-independent method for determining Sleeping Beauty transposon copy numbers.

    Mob DNA 2011, 2:5. PubMed Abstract | BioMed Central Full Text | PubMed Central Full Text OpenURL