Email updates

Keep up to date with the latest news and content from Mobile DNA and BioMed Central.

Open Access Short Report

Insertion site preference of Mu, Tn5, and Tn7 transposons

Brian Green1, Christiane Bouchier2, Cécile Fairhead3, Nancy L Craig4 and Brendan P Cormack1*

Author Affiliations

1 Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Hunterian 617, 725 North Wolfe Street, Baltimore, MD 21205-2185, USA

2 Institut Pasteur, Plate-forme Génomique, Département Génomes et Génétique, Rue du Dr. Roux, F-75015 Paris, France

3 Institut de Génétique et Microbiologie, Université Paris Sud 11, CNRS UMR8621, 15 rue Georges Clémenceau, Orsay F-91405, France

4 Department of Molecular Biology and Genetics, The Howard Hughes Medical Institu725 North Wolfe Street Johns Hopkins University School of Medicine, Baltimore, MD 21205-2185, USA

For all author emails, please log on.

Mobile DNA 2012, 3:3  doi:10.1186/1759-8753-3-3

Published: 7 February 2012

Abstract

Background

Transposons, segments of DNA that can mobilize to other locations in a genome, are often used for insertion mutagenesis or to generate priming sites for sequencing of large DNA molecules. For both of these uses, a transposon with minimal insertion bias is desired to allow complete coverage with minimal oversampling.

Findings

Three transposons, Mu, Tn5, and Tn7, were used to generate insertions in the same set of fosmids containing Candida glabrata genomic DNA. Tn7 demonstrates markedly less insertion bias than either Mu or Tn5, with both Mu and Tn5 biased toward sequences containing guanosine (G) and cytidine (C). This preference of Mu and Tn5 yields less uniform spacing of insertions than for Tn7, in the adenosine (A) and thymidine (T) rich genome of C. glabrata (39% GC).

Conclusions

In light of its more uniform distribution of insertions, Tn7 should be considered for applications in which insertion bias is deleterious.

Keywords:
Tn7; Mu; Tn5; Mutagenesis; Insertion site; DNA transposon; Mobile element