Table 2 |
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|
Integration site analysis for remobilized progeny from 7M Hoppers |
||||
|
Tadpole |
Right IR/DR flanking sequence |
Integration site |
5' flanking gene |
3' flanking gene |
|
|
||||
|
Parental 7M |
TGTTAGTTATTACTTAcagttgaagtcgg |
38:3796832 |
EDEM2 |
PHF20 |
|
|
||||
|
E208-91 |
TCATTATCAGTATATAcagttgaagtcgg |
38:552620 |
EMILIN3 |
EMILIN3 |
|
3-1 |
GCTACTCACACAGTTAcagttgaagtcgg |
13:899336 |
SPRY2 |
NDFIP2 |
|
2-7M |
TGTTACTGGGCACTTAcagttgaagtcgg |
3:3883685 |
MYNN |
MDS1 |
|
A21A-1 |
AGTGTGTAGCTATATAcagttgaagtcgg |
909:49452 |
GBP3 |
GBP3 |
|
C2AB-5 |
CGGGCCATGATGTATAcagttgaagtcgg |
Repeat |
- |
- |
|
E2F3-95 |
GCACATAATACACATAcagttgaagtcgg |
Unknown |
- |
- |
|
E220-248 |
CATGTAcagttgaagtcgg |
Unknown |
- |
- |
|
|
||||
|
Sequences flanking seven novel transposon insertion sites from 7M hoppers were cloned using extension primer tag selection linker mediated-PCR. The transposon sequence (italics) is flanked by the characteristic TA dinucleotide target site duplication (bold). The flanking sequence (depicted in capital letters) was used to interrogate the X. tropicalis genome sequence database (Joint Genome Institute X. tropicalis genome sequence assembly v4.1; [3]) to assign the integration sites to the genomic sequence scaffolds. The genes flanking the novel integration site are indicated. |
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|
Yergeau et al. Mobile DNA 2011 2:15 doi:10.1186/1759-8753-2-15 |
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