 ResearchImmunity of replicating Mu to self-integration: a novel mechanism employing MuB proteinJun Ge Section of Molecular Genetics and Microbiology and Institute of Cellular and Molecular Biology, University of Texas at Austin, Austin, TX 78712, USA
Mobile DNA 2010, 1:8doi:10.1186/1759-8753-1-8
Additional filesAdditional file 1: Figure S1. (a) Lysis profiles of various Mu lysogens. All cultures were grown at 30°C to an OD600 nm of 0.6, shifted to 42°C for 40 minutes for phage induction, and shifted down to 37°C until lysis. 0 indicates time of temperature shift-up. Δhns strain did not lyse naturally, so lysis was induced by addition of chloroform. Phage titers from all three strains were similar to those from the parent strain MP1999, yielding around 3 to 5 × 108 pfu/ml. ChIP samples were prepared at 40 minutes for all SJG3-derived strains. (b) Western blot of MP1999 with anti-MuB antibodies at indicated times after temperature shift-up. Format: JPEG Size: 848KB Download file  Additional file 2: Figure S2. Moving average AT-content of Mu genome. The window for average AT-content (%) calculation is 100 bp and the moving step is 20 bp. The value of AT content at a given position represents the average AT content of 100 bp DNA starting from that position. The graph was derived from data in [20]. Format: JPEG Size: 1MB Download file Additional file 3: Table S1. Sequence of primers used in this study. Format: DOCX Size: 15KB Download file |
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