Research
Transposable elements in a marginal plant population: temporal fluctuations provide new insights into genome evolution of wild diploid wheat
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* Corresponding authors: Alexander Belyayev belyayev@research.haifa.ac.il - Alan H Schulman alan.schulman@helsinki.fi
1 Institute of Evolution, University of Haifa, Mount Carmel, Haifa, Israel
2 MTT/BI Plant Genomics Laboratory, Institute of Biotechnology, Viikki Biocenter, University of Helsinki, Helsinki, Finland
3 Plant Genomics, Biotechnology and Food Research, MTT Agrifood Research, Jokioinen, Finland
Mobile DNA 2010, 1:6 doi:10.1186/1759-8753-1-6
Published: 1 February 2010Additional files
Additional file 1:
Transposable element (TE) copy numbers in three self-pollinated generations of three genotypes from the Kishon population of Ae. speltoides.
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Additional file 2:
Various. Supplementary methods. Quantitative real-time polymerase chain reaction (PCR) and relative quantification. Long distance PCR for transposable elements (TEs). Dot blot hybridization and analysis. Inter-retrotransposon amplified polymorphism (IRAP) for TE transposition. Cloning and sequencing of unique IRAP bands. Chromosome spread preparation, probe labeling, in situ hybridization, detection, and differential staining procedures. Table S1. Relative TE copy numbers in sibs of explored genotypes. Table S2. Sequences with new TE insertions. Table S3. Transposable element accessions. Table S4. Specific primers and primer sets for Aegilops speltoides TEs, 5S ribosomal RNA (rRNA) genes and Spelt 52 tandem repeats. Table S5. Primer combinations for quantitative PCR. Table S6. Primers for IRAP analysis. Table S7. The significance of deviations in each generation via the t-test and non-parametric Mann-Whitney U test.
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