Figure 2.

Excision frequency in yeast catalyzed by wild type and mutant Ping and Pong proteins. Histograms showing average excision frequencies for mPing (A) and mPong (B) in yeast using different combinations of Ping and Pong proteins. TPase - nuclear export signal (NES) mutants contain alanine substitutions at the two conserved leucines in the predicted NES (Ping 384+386, Pong 418+420). Error bars represent the standard error. Sequence of the NES for the Ping and Pong TPase proteins are shown in the top left corner. Residues that match the consensus NES are in red.