Transposition of the Tourist-MITE mPing in yeast: an assay that retains key features of catalysis by the class 2 PIF/Harbinger superfamily

doi:10.1186/1759-8753-1-5

Mobile DNA

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Transposition of the Tourist-MITE mPing in yeast: an assay that retains key features of catalysis by the class 2 PIF/Harbinger superfamily

C Nathan Hancock¹ , Feng Zhang¹^,2 and Susan R Wessler¹

¹Plant Biology Department, University of Georgia, Athens, GA 30602, USA

²Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, MN 55455, USA

author email corresponding author email

Mobile DNA 2010, 1:5doi:10.1186/1759-8753-1-5


Published:	1 February 2010

Additional files

Additional file 1:

Table of yeast excision frequency data. Excel file containing the data set used to produce Figure 2. Frequency = ADE2 revertants/total cells plated.

Format: XLSX Size: 20KB Download file

Additional file 2:

NetNES output. PDF file containing the prediction of the nuclear export signal (NES) signal in the C-terminal region of the Ping TPase protein. NN indicates the neural network score, HMM indicates the hidden Markov model score. Scores above the threshold indicate potential NES signals.

Format: PDF Size: 8KB Download file

This file can be viewed with: Adobe Acrobat Reader

Additional file 3:

Insertion site sequences. Excel file containing a table of the mPing and mPong insertions in yeast.

Format: XLS Size: 26KB Download file

This file can be viewed with: Microsoft Excel Viewer