Table 5

Yeast 2 hybrid assay shows that TnsC and TnsD can interact in vivo.

Protein fragment

β-galactosidase activity

Fos

Jun

5.0

E2F1

RB

1.0

Empty vector

Gal4-AD

Empty vector

Gal4-DB

0.075

TnsC (1 to 555) wt

TnsD (1 to 507) wt

1.0

TnsC (1 to 555) wt

TnsD (1 to 436)

0.075

TnsC (1 to 555) wt

TnsD (1 to 399)

4.5

TnsC (1 to 555) wt

TnsD (1 to 375)

5.0

TnsC (1 to 555) wt

TnsD (1 to 350)

4.5

TnsC (1 to 555) wt

TnsD (1 to 309)^a

4.5

TnsC (1 to 555) wt

TnsD (1 to 293)

0.075

TnsC (1 to 555) wt

TnsD (8 to 507)

0.075

TnsC (1 to 555) wt

TnsD (12 to 507)

0.075

TnsC (1 to 555) wt

TnsD (16 to 507)

0.075

TnsC (1 to 555) wt

TnsD (22 to 507)

0.075

TnsC (1 to 555) wt

TnsD (1 to 507) wt

1.0

TnsC (1 to 480)

TnsD (1 to 507) wt

1.0

TnsC (1 to 384)

TnsD (1 to 507) wt

1.0

TnsC (1 to 332)

TnsD (1 to 507) wt

0.20

TnsC (1 to 293)^a

TnsD (1 to 507) wt

0.20

TnsC (1 to 280)

TnsD (1 to 507) wt

0.014

^aDeletion analysis of TnsD and TnsC revealed that this protein-protein interaction activity is contained within TnsC 1 to 293 and TnsD 1 to 309.

Mitra et al. Mobile DNA 2010 1:18   doi:10.1186/1759-8753-1-18