Figure 1.

Interaction of Ty3 Gag3 subdomains shown by interactions between Gal4 DNA binding domain (BD) and activation domain (AD) fusions to Ty3 Gag3 subdomains. Interaction restores Gal4 mediated activation of ADE2 under control of the GAL1-10 upstream activating sequences (UASs) and growth on medium lacking adenine. Cells expressing ADE2 have reduced accumulation of a red adenine biosynthetic intermediate. Yeast strain yAH109 was transformed with two-hybrid pGAD or pGBK vectors (V) or expression plasmids. Streaks are representative of independent transformants growing on synthetic dextrose medium lacking tryptophan, leucine, and adenine. Gal4-BD fusions are labeled in the top row and shown in columns. Gal4-AD fusions are labeled in the left column and shown in rows. Scoring is based on four individual transformants. The most variability was observed among individual transformants expressing Gag3 and capsid (CA)-N-terminal domain fusions.